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  • Product Name:

    Acute HS-Taq DNA Polymerase

  • Type:
    Molecular Diagnostics
  • Code:
    CSB-DEM024
  • Description:

    The hot-start versions ofCUSAg Taq DNA Polymerase contain a mixture of Taq polymerase(Cat:CSB-DEM023) and a monoclonal antibody(Cat:CSB-DA403AmN①) that binds to Taq polymerase, thereby preventing DNA synthesis at room temperature. During the initial DNA denaturation step (reaction temperature ~94°C), the antibody is denatured, releasing the polymerase and allowing DNA synthesis to proceed. The use of a hot-start PCR enzyme prevents nonspecific amplification due to mispriming and/or the formation of primer dimers during PCR assembly.

  • Purity:
    90%±5% by SDS-PAGE
  • Shape:
    Liquid
  • Storage:
    Long term storage at -20 °C, short term storage at 4 °C
  • Source:
    Microorganism
  • EC No.:
    EC 2.7.7.7
  • Active:
    5 U/μL
  • Activity is defined:
    One unit is defined as the amount of polymerase that incorporates 10 nmoles of  dNTPs into acid-precipitable DNA in30 minutes at 72 °C under standard assay  conditions. 
  • Application:
    qPCR, qRT-PCR