Trypsinogen-2 (PRSS2) is a 25 kDa pancreatic proteinase, encoded by the PRSS2 gene.PRSS2 are secreted at high concentrations into pancreatic fluid, but a small proportion escapes into the blood circulation. PRSS2 exists in  pancreas with inactive zymogen, which is
activated by enterokinase.

When pancreatitis were damaged, the concentration of PRSS2 is high in the blood circulation. Because of RSS2 relatively small size, PRSS2 are readily filtered through the glomeruli. For unknown reasons, the tubular reabsorption of PRSS2 is lower, and consequently, the urinary concentration of PRSS2 is high. Measurement of urinary PRSS2 is considered useful in diagnosing acute pancreatitis and assessing its severity.