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  • Product Name:

    RNase Inhibitor

  • Type:
    Molecular Diagnostics
  • Code:
    CSB-DEM029
  • Description:
    RNase Inhibitor specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1
    ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from
    Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).

    The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 1014.


    Instructions

    Protocol for Avoiding RNase Contamination using RNase Inhibitor

    Add inhibitor to achieve final concentration of 1U/ul in your reaction. During assembly of a reaction, RNase
    Inhibitor should be added before other components that are a possible source of RNase contamination (i.e.

    enzymes, plasmid from a mini prep.)


    *RNase Inhibitor is a 50 kDa protein that will be inactivated in denaturing conditions.

    **RNase Inhibitor should be used below 50°C.


    Molecular Weight

    50 kDa


    Storage buffer

    20 mM HEPES-KOH, 50 mM KCl, 8 mM DTT, 50% Glycerol, pH7.6


    Quality Control

    RNase Inhibitor is tested for contaminating activities, with no trace of endonuclease activity, nicking activity or exonuclease


    Heat Inactivation

    Yes


    Notes

    Since ribonucleases typically retain activity under denaturing conditions, care must be taken to avoid denaturing RNase Inhibitor molecules which have complexed with a ribonuclease. To prevent the release of active ribonuclease, temperatures greater than 50°C and high concentrations of urea or other denaturing agents should be avoided.

    The recommended concentration of RNase Inhibitor in a reaction is 1 U/μl. During assembly of a reaction,
    RNase Inhibitor should be added before other components that are a possible source of RNase contamination (i.e. enzymes, plasmid from a mini prep.)
  • Shape:
    Liquid
  • Storage:
    Long term storage at -20 °C, short term storage at 2-8 °C
  • Source:
    Recombinant in E.coli
  • Active:
    40 U/μL
  • Activity is defined:
    One unit is defined as the amount of RNase Inhibitor, Mammalian required to inhibit the activity of 5ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A.
  • Application:
    cDNA synthesis, In vitro translation/transcription, RT-PCR
  • Size:
    2000 U or user-defined