Biochemical Diagnostic Materia
Enzymes are a special type of protein that can catalyze chemical reactions in the body. People have used the determination of enzyme activity in body fluids for clinical diagnosis for more than a century. Biochemical diagnostic enzymes are mainly used to establish biochemical diagnostic detection systems. This system is based on the principle of enzyme-catalyzed cascade reaction and is used for enzyme activity detection of specific functions of the human body to achieve monitoring of various indicators.
The biochemical enzyme products independently developed and produced by Cusag have been repeatedly verified by biochemical analysis platforms for their enzymatic properties, solid stability, and applicability analysis. They have been compared with well-known high-quality reagent products on the market. Under the same conditions, multiple groups of random samples were measured. It was found that there was good clinical correlation between the two and could be used for clinical biochemical in vitro diagnosis.
Biochemical Diagnostic Materia Product Category
Pyranose Oxidase (PROD)
Pyranose oxidase (PROD, EC 220.127.116.11) is a type of glucose 2-oxidase produced by microorganisms in the Basidiomycota. 1,5-anhydroglucitol (1,5-AG) is the C-1 deoxy form of pyranose glucose and has received much attention in the field of sugar metabolism as a new indicator for evaluating blood glucose control recently proposed by the academic community.
Pyranose oxidase, glucose kinase and ATP regeneration system can detect changes in the concentration of serum 1,5-AG. Glucose kinase and ATP regeneration system can effectively convert glucose into a compound that does not react with pyranose oxidase, glucose-6-phosphate, and this method can selectively target changes in 1,5-AG. Hydrogen peroxide generated by the oxidation of 1,5-AG by pyranose oxidase can be detected by the TRINDER reaction.
Hexokinase (HK, EC 18.104.22.168) is widely present in animals, plants, microorganisms and cultured cells. It is the first key enzyme in glucose metabolism and catalyzes the conversion of glucose to 6-phosphogluconate. 6-phosphogluconate is the intersection of glycolysis and pentose phosphate pathway.
The glucose oxidase (GOD) method and hexokinase (HK) method are commonly used in clinical laboratories to determine glucose. The hexokinase method is currently recognized as the reference method for determining glucose internationally due to its strong specificity, high precision and accuracy. Mild hemolysis, lipemia, jaundice, vitamin C, sodium fluoride, liver disease, EDTA and oxalate do not interfere with this method.
Acetyl-coenzyme A Synthetase (Acs)
In the detection of free fatty acids (NEFA), Acyl-CoA synthetase (ACS, EC 22.214.171.124) is the key raw material for the first step of enzymatic reaction. Under the condition that free fatty acids in the sample coexist with coenzyme A and adenosine triphosphate (ATP), lipoyl CoA, AMP and pyrophosphate are generated based on the action of ACS.
Acyl-CoA oxidase (ACO)
In the detection of free fatty acids (NEFA), Acyl-CoA oxidase (ACO, EC126.96.36.199) is the key raw material for the second step of enzymatic reaction. Acyl-CoA oxidase (ACO, EC188.8.131.52) is mainly used to oxidize acyl-CoA to generate 2,3-trans-enoyl-CoA and hydrogen peroxide. Hydrogen peroxide can also be used for quantitative detection of NEFA under the action of peroxidase.
Cystathionine beta-synthase (CBS)
Cystathionine β-synthase (CBS, EC 184.108.40.206) is a homologous tetramer in the cytoplasm composed of 63KDa subunits and is a pyridoxal phosphate (PLP)-dependent enzyme. Under the catalysis of vitamin B6, CBS can synthesize cystathionine by combining serine with homocysteine (Hcy).
Cystathionine beta-lyase (CBL)
Cystathionine beta-lyase (CBL, EC220.127.116.11) belongs to the lyase class and catalyzes the cleavage of cystathionine at the β position, generating homocysteine after removal of pyruvic acid and NH3. CBS and CBL are used for the detection of homocysteine in blood in enzyme cycling methodology.
Creatininase amidohydrolase (CAH)
Creatinine is one of the important indicators reflecting renal function. Due to the strong specificity of enzyme-catalyzed reactions, it can greatly improve the reliability of the measurement results and is the development direction of creatinine measurement. Creatininase (CAH, EC 18.104.22.168) can convert creatinine into creatine, which is the first step in creatinine enzyme detection.
Creatine amidinohydrolase (CRH)
Creatinase (CRH, EC 22.214.171.124) mainly hydrolyzes creatine to generate sarcosine and urea, which is the second step in creatinine enzyme detection.
Sarcosine oxidase (SOX)
Sarcosine Oxidase (SOX, EC 126.96.36.199) can specifically catalyze the substrate sarcosine to generate glycine and is the third step in creatinine enzyme detection.
Malate dehydrogenase (MDH)
Malate dehydrogenase (MDH, EC 188.8.131.52) is widely present in animals, plants and microorganisms and can catalyze the reversible conversion between oxaloacetate and malate. MDH can catalyze the generation of malate from oxaloacetate, generating NADH from NAD+, providing reducing power for nitrate reduction. In serum, bicarbonate reacts with phosphoenolpyruvate (PEP) under the catalysis of phosphoenolpyruvate carboxylase (PEPC) to generate oxaloacetate and phosphate; oxaloacetate is generated from malate dehydrogenase (MDH) under the catalysis of malate dehydrogenase (MDH), generating malic acid at the same time, while NADH is oxidized to NAD+. MDH can be used as a key raw material for serum CO2 detection.
Purine-nucleoside phosphorylase (PNP)
Purine nucleoside phosphorylase (PNP or PNPase, EC184.108.40.206) is a key enzyme in the purine salvage synthesis pathway and is widely present in prokaryotes and eukaryotes. The enzyme catalyzes the reversible hydrolysis of purine nucleoside phosphates, breaking down the substrate purine nucleoside into the corresponding base and ribose-1-phosphate. In vitro reactions can synthesize new purine nucleosides or analogs by adding another purine base or its analogs. Currently, it is mainly used for 5'-nucleotidase (5'-NT) and adenosine deaminase (ADA) detection.