Cytokine

Leukemia suppressor factor (LIF) was discovered in the late 1960s to induce the differentiation of mouse myeloid leukemia cell M1 into normal M-like cells. After its cloning in 1987, According to its amino acid sequence, it was found to be the same substance with some previously discovered substances such as hepatocyte stimulator factor-I (HF-I), human interleukin differentiation factor (hILDA) and cholinergic nerve growth factor (CNGF). The human and mouse LIF genes were located on chromosome 22 and chromosome 11, respectively. The length of the genes was 6.0kb and 6.3kb, respectively, containing 3 exons and 2 introns. The coding region of the genes was highly conserved, with homology ranging from 78 to 94%. With 7 glycosylation sites and 6 Cys, the intramolecular disulfide bond may play an important role in maintaining the structure and biological activity of LIF molecules. LIF and its receptor (LIFR) are distributed in different tissues and cells, and have a wide range of biological effects, including participating in hematopoietic regulation, inducing normal differentiation of M1 cells in vitro, and inhibiting embryo-to-cell (ES) differentiation.

Human leukemia suppressor is a lymphoid factor that maintains the long-term survival of embryonic stem cells by inhibiting spontaneous differentiation. The sequence homology of mature LIF in human and mouse is 78% at the amino acid level. Human LIF is equally active in human and mouse cells, and the activity of mouse LIF in human cells is about 1000 times lower than that of human LIF. The recombinant human LIF produced by our company is expressed by prokaryotic expression system expressing human LIF gene, and purified by separation and purification. To avoid repeated freezing-thawing, it is recommended to pack recombinant human LIF protein into small portions and use one dose at a time.

Interleulin-4 (IL-4) is a cytokine activated by Th2 cells with a molecular weight of 15kd. IL-4 has a variety of biological effects and plays an important role in the proliferation, differentiation and functional regulation of T cells, B cells and macrophages. According to the literature, in the allergic diseases such as scleroderma, multiple sclerosis, autoimmune thyroid disease, inflammatory bowel disease, bronchial asthma, atopic dermatitis, four levels of the body are significantly increased. This kit uses self-made free serum IL-4 and IL-4 to determine the IL-4 levels in human serum, body fluid or their culture supernatant, which can assist the diagnosis of clinical diseases and provide a reliable preventive means for further exploration of its mechanism of action and the pharmacology of various clinical systems. The recombinant rabbit IL4 produced by our company was expressed by prokaryotic expression system and purified by separation and purification.

Interleukin 21 (IL-21) is a type I cytokine that is secreted by activated CD4 + T cells and NKT cells and has a four-molecule spiral structure similar to that of IL-15. IL-21 is an important immune molecule. Through its receptor (IL-21r) and specific binding, IL-21 is distributed in the entire lymphohematopoietic system and some non-gonorrhea tissues, mainly in the cell membranes of B cells, natural killer cells and some T cells. IL-21 is involved in the regulation of B cell proliferation, and IL-15 collaboratively promotes the proliferation, differentiation and cell activity of bone marrow progenitor cells and NK cells. The recombinant rabbit IL21 produced by our company was prepared by prokaryotic expression system, separation and purification.

Interleukin-3 (IL-3) stimulates the proliferation and differentiation of pluripotent stem cells and multiple progenitors, also known as multi-colony stimulating factor, multi-CSF and hemopoietic cell growth factor (HCGF) human IL-3 is localized in chromosome 5 (5q23-31), which may be related to the synergistic regulation of hematopoietic process. It may also be associated with the morbidity of MDS, ANLL, refractory megaloblastic anemia and other diseases. This product is made by the prokaryotic expression system (E.coli) containing highly expressed human IL-3 gene after fermentation, isolation and highly purified, and then freeze-dried. The polypeptide chain was composed of 133 amino acid residues with a molecular weight of 15.0kDa.

IL-6, also known as B-cell stimulator 2(BSF-2, BCDF), interferon-β2 (IFN-β2), hybridoma/plasma cell tumor growth factor (H (P) GF) and hepatocyte stimulator, is a kind of cytokine, belonging to a kind of interleukin. It can be produced by a variety of cell types, such as T cells, monocytes, fibroblasts, endothelial cells, etc. IL-1, TNF-a, PDGF, viral infection, double-stranded RNA and cAMP can induce the production of interleukin-6 in normal cells. Interleukin-6 stimulates the proliferation, differentiation and function of cells involved in the immune response. It can induce B cell differentiation, support plasmacytoma and myeloma proliferation, and induce monocyte differentiation. Enhance the activity of NK cells, induce the acute phase reaction molecules, stimulate hepatocytes and induce neuronal differentiation. It has been found that IL-6 and IL-3 cooperate to support hematopoietic stem cell proliferation (hematopoietic activity). The product is expressed by prokaryotic system, purified by separation, bactericidal filtration and other steps, with a molecular weight of 22.5kDa.

Human fibronectin FN (CH296) is a high molecular weight glycoprotein that exists in body fluids in either a soluble form or in the extracellular matrix in an insoluble form. As one of the main cell adhesion molecules, FN can regulate cell shape and cytoskeleton organization through cell signal transduction pathway, and promote cell spread. In addition, FN plays a key role in many important physiological processes, such as embryo formation, wound healing, hemostasis and thrombosis. The functional domain of FN was used as the target gene, and the expression plasmid was constructed and introduced into E.coli strain for large amounts of expression. After a series of purification, recombinant human fipronectin (rhFN) with molecular weight of 63kDa was obtained. The rhFN consists of two cell adhesion domains (C-Domain and CS-1) and one virus adhesion domain (H-Domain), which can not only effectively improve the adhesion and proliferation ability of cells, but also greatly enhance the efficiency of lentivirus, retrovirus and other target cells to infect.

Bone morphogenetic protein (BMPs) is a member of transforming growth factor β (TGF-β) superfamily. Since Urist and other scholars first extracted a protein component that is helpful to bone formation from decalcified bone in 1965, a variety of bone morphogenetic proteins have been found successively. And found its role in promoting osteogenesis. Among them, human bone morphogenetic protein-4 (BMP-4), as a member of the BMPs family, is consistent with the general structure of BMPs in spatial structure, but has its specificity only in the mature region sequence. BMP-4 plays an important role in promoting bone tissue regeneration and repair. In addition, BMP4 is also closely related to inducing embryonic differentiation, guiding neural stem cell differentiation, regulating tumor growth and invasion, and some cardiovascular and cerebrovascular diseases. This product is a human recombinant protein prepared by E. coli prokaryotic expression system.