Pfu Ⅱ high fidelity DNA polymerase
Product Introduction
Pfu II DNA Polymerase is derived from Pfu II DNA Polymerase through genetic engineering. It is a highly thermostable DNA polymerase obtained by recombinant expression in E. coli, fused with a continuous capacity-enhancing structural domain, and has high amplification efficiency and fidelity, used for amplification of difficult templates. The amplification efficiency can reach a speed of 1kb/10s, and it also has strong long fragment amplification capability.
Product Feature
- High purity: Purity >95%, no residual nuclease, low residual host gDNA.
- Good fidelity: Higher fidelity compared to Taq DNA Polymerase.
- High sensitivity: Improved template affinity through modification, increasing the detection sensitivity of low concentration templates.
- Long amplification fragment: Can amplify λ-DNA target fragments up to 10kb in length.
Product Application
- Long fragment PCR amplification
- Cloning of DNA fragments
Product Data
SDS-PAGE electrophoresis image
Figure 1. SDS-PAGE analysis of CSB-DEM043 Pfu II DNA Polymerase.
Amplification effect at different template concentrations.
M: Trans2K DNA Marker, NC: Negative control.
Figure 2. CSB-DEM043 Pfu II DNA Polymerase amplifies 1kb fragment of λ-DNA, and the target fragment can be effectively amplified at different template amounts (100ng-1pg), with a sensitivity as low as 1pg.
Comparison test with competitive enzymes.
Line 1-6: Pfu II DNA Polymerase, λ-DNA 1/2/4/6/8/10Kb.
Line 7-12: Brand N Pfu II DNA Polymerase, λ-DNA 1/2/4/6/8/10Kb.
M: Trans15K DNA Marker.
Figure 3. CSB-DEM043 Pfu II DNA Polymerase amplifies λ-DNA fragments of 1-10kb, compared to a certain brand of Pfu II DNA Polymerase.