Molecular Biology

Pfu Ⅱ high fidelity DNA polymerase

Catalogue #
1 U/µL,100 U; 1 U/µL,500 U; 1 U/µL,1000 U;
Product Specification:

Product Introduction

Pfu II DNA Polymerase is derived from Pfu II DNA Polymerase through genetic engineering. It is a highly thermostable DNA polymerase obtained by recombinant expression in E. coli, fused with a continuous capacity-enhancing structural domain, and has high amplification efficiency and fidelity, used for amplification of difficult templates. The amplification efficiency can reach a speed of 1kb/10s, and it also has strong long fragment amplification capability.

Product Feature

- High purity: Purity >95%, no residual nuclease, low residual host gDNA.

- Good fidelity: Higher fidelity compared to Taq DNA Polymerase.

- High sensitivity: Improved template affinity through modification, increasing the detection sensitivity of low concentration templates.

- Long amplification fragment: Can amplify λ-DNA target fragments up to 10kb in length.

Product Application

- Long fragment PCR amplification

- Cloning of DNA fragments

Product Data

SDS-PAGE electrophoresis image

Figure 1. SDS-PAGE analysis of CSB-DEM043 Pfu II DNA Polymerase.



Amplification effect at different template concentrations.

M: Trans2K DNA Marker, NC: Negative control.

Figure 2. CSB-DEM043 Pfu II DNA Polymerase amplifies 1kb fragment of λ-DNA, and the target fragment can be effectively amplified at different template amounts (100ng-1pg), with a sensitivity as low as 1pg.


Comparison test with competitive enzymes.

Line 1-6: Pfu II DNA Polymerase, λ-DNA 1/2/4/6/8/10Kb.

Line 7-12: Brand N Pfu II DNA Polymerase, λ-DNA 1/2/4/6/8/10Kb.

M: Trans15K DNA Marker.

Figure 3. CSB-DEM043 Pfu II DNA Polymerase amplifies λ-DNA fragments of 1-10kb, compared to a certain brand of Pfu II DNA Polymerase.