Seamless Cloning Kit

Product Introduction

Seamless Cloning Kit is a simple, fast, and efficient DNA directional cloning product that can quickly, efficiently, and accurately insert one or more DNA fragments into a linearized vector in a predetermined direction through homologous recombination. This kit has no restriction on enzyme cutting sites when performing gene cloning. It only needs to linearize the vector at the predetermined cloning site by enzyme cutting or directly prepare the linearized vector by PCR method. The 5' end of the two PCR primers of the inserted fragment introduces a 15-25bp overlapping sequence that is completely consistent with both ends of the vector cloning site. Only one-step recombination is needed to obtain a high-efficiency cloned recombinant vector.

Principle of action:

Product Feature

- Flexible site selection: gene cloning at any position of the vector;

- Fast and simple: vector construction can be completed within an hour;

- High cloning efficiency, positive cloning up to 90% or more;

- Recombination of multiple target genes at once;

Product Application

- Rapid cloning;

- High-throughput cloning;

- Seamless cloning;

- DNA site-directed mutagenesis;

Product Data

Figure 1. Recombinant transformation plate.

Figure 2. Colony PCR identification of the clones constructed.

M：Trans2K DNA Marker