Uracil-DNA Glycosylase（UDG）
Product Introduction
UDG enzyme, uracil-DNA glycosylase, is a recombinant protein purified from cold-loving marine bacteria and expressed in E. coli strains. It can hydrolyze the N-glycosidic bond between the uracil base and the sugar-phosphate backbone of dU-containing single-stranded or double-stranded DNA, releasing free uracil and forming a DNA chain with missing bases that is easily hydrolyzed and broken. The enzyme can be inactivated at temperatures above 50°C, making it suitable for PCR, qPCR and other systems.
Product Feature
UDG enzyme, uracil-DNA glycosylase, is a recombinant protein purified from cold-loving marine bacteria and expressed in E. coli strains. It can hydrolyze the N-glycosidic bond between the uracil base and the sugar-phosphate backbone of dU-containing single-stranded or double-stranded DNA, releasing free uracil and forming a DNA chain with missing bases that is easily hydrolyzed and broken. The enzyme can be inactivated at temperatures above 50°C, making it suitable for PCR, qPCR and other systems.
Product Application
- PCR, RT-PCR
- qPCR, RT-qPCR, etc.
Product Data
SDS-PAGE electrophoresis
Figure 1. SDS-PAGE analysis of CSB-DEM026 UDG enzyme.
Enzyme action effect.
Figure 2. CSB-DEM026 UDG enzyme action result chart.
Note: Incubation at 25°C for 10 min activates UDG to degrade U-containing products and reduce the chance of false positive signals. The Ct values of three groups of reactions with and without heat-sensitive UDG were calculated, and the larger the Ct value, the higher the efficiency of removing residual products. This experiment shows that 0.5U/reaction of this heat-sensitive UDG can effectively degrade U-containing products and has good ability to remove residual templates.